NcoI - NEB
WEBNcoI has a High Fidelity version NcoI-HF ® (NEB #R3193). High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.
NcoI-HF - NEB
WEBHigh Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.
Compatible Cohesive Ends and Generation of New Restriction Sites | NEB
WEBNew restriction sites can be generated by ligation of DNA fragments with compatible cohesive or blunt ends. These new restriction sites may be generated by: Cleavage followed by fill-in of 5´ overhangs to generate blunt ends. Cleavage with two restriction endonucleases that produce blunt ends.
NotI-HF - NEB
WEBHigh Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity.
NEBuffer Activity/Performance Chart with Restriction Enzymes | NEB
WEBNEB’s restriction enzyme buffer system makes your restriction digests easy and convenient. We are able to offer >210 restriction enzymes that cut in a single buffer, rCutSmart™ . This improves ease-of-use, especially when performing double digests.
Restriction Endonucleases Products | NEB
WEBOver 210 restriction enzymes are 100% active in a single buffer – rCutSmart™ Buffer. >180 restriction enzymes are Time-Saver qualified, meaning you can digest DNA in 5-15 minutes, or digest DNA safely overnight. Choose from >265 restriction enzymes, the largest selection commercially available.
Double Digest Protocol with Standard Restriction Enzymes | NEB
WEBMar 24, 2021 · Digesting a DNA substrate with two restriction enzymes simultaneously (double digestion) is a common timesaving procedure. Over 210 restriction enzymes are 100% active in rCutSmart™ Buffer, making double digestion simple.
Restriction Digest Protocol - NEB
WEBJul 30, 2018 · A specific protocol for single digestion using this restriction enzyme can be accessed using our free online tool, NEBcloner. Please note that NEBcloner will also provide detailed double digest protocols using this enzyme.
Double Digests - NEB
WEBThe Performance Chart for Restriction Enzymes rates the percentage activity of each restriction endonuclease in the four standard NEBuffers. NEB’s online tools, NEBcloner and Double Digest Finder will help guide your reaction buffer …
NEBcutter V2.0
WEBNEBcutter V2.0. This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut the sequence just once.
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